Quickly get sections as slim or thick as your application involves, without worrying about dropping or distorting precious tissue samples.
Megatome is really a vibrating microtome created to part a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables higher-throughput tissue sectioning with uniform surface area profile, and also small tissue injury and data reduction.
Antibodies may well acquire months to diffuse as a result of only a few millimeters of tissue, with a steep labeling gradient from surface area to Main.
Running the CLI directly from a Gradle endeavor is not really now supported. A distribution needs to be made by means of gradlew :j2d-cli:distZip to produce a zip file that contains almost everything necessary to run.
SE utilizes a rotational electric field to disperse really electromobile molecules (like antibodies or surfactant micelles) all through a porous sample with out damaging electrically billed constructions inside the tissue. This enables 2-four working day clearing of intact organs,
Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them absolutely clear. This permits light penetration into your sample and makes certain the acquisition of substantial-resolution, in-aim impression info.
Megatome can be a novel microtome that allows for top-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with minimal tissue problems and data decline.
It’s time to update your microtome to Megatome. With accurate significant-frequency slicing for an unmatched selection of sample dimensions and types – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for varied programs.
Thoroughly delipidate whole mouse brains or comparably sized samples in only one working day with SmartBatch+, or in one week with our passive clearing package.
Docset generation calls for at minimum two possibilities: the identify in the docset and The situation from the Javadoc documents to include while in the docset.
This will likely make a docset named Sample in the current Listing. Docset generation may be tailored with optional arguments:
SHIELD avoids the variability of hydrogel embedding and the knowledge loss from PFA preservation, shielding specimens for multiple rounds of processing.
--displayName: Will established the identify as shown in Dash. That is helpful if you produce a docset with title SampleProject but Display screen name Sample Venture alternatively. This location will default to the value of --title if omitted.
Our novel Protect tissue preservation technique types intramolecular bonds employing megatomi.com polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.